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Dec 28, 2019 Rotor-Gene Q (Rotor-Gene.exe). Rotor-Gene Q is a program designed for the QIAGEN Rotor-Gene Q device that enables streamlined analysis for a wide range of applications. It combines multiple optimized features to provide powerful performance.
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Apple Software Update is a software tool by Apple that installs the latest version of Apple software. It was originally introduced to Mac users in Mac OS 9. A Windows version has been available since the introduction of iTunes 7. more info... - More
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REST2009 is a popular bioinformatics tool to analyze relevant geneexpression changes from crossing points (CP) of amplification curvesgathered by a Real-Time PCR (qPCR) experiment. REST was developed byMichael W. Pfaffl (Technical University Munich) and QIAGEN.
However,I found REST 2009 (v2.0.13) to cease working when moving from a 32bitWindows operating system (Windows XP) to a 64bit Windows operatingsystem (Windows 7). The accepted solution until now was, to install aVirtualPC software including a Windows XP image optimized to run underWindows 7 (dubbed Windows XP Mode). Resourcewise it was a disaster, notto mention the hassle of backing up an entire second system. Also, theneed for additional security software in your virtual image made thesystem even slower, remember Microsoft ceased to provide securityupdates for Windows XP from the 8th of April 2014. Occasionally yourwould need need to install other 3rd party software to complete youranalyses, in my case, I needed to install a PDF printer to be able tosave my REST 2009 reports in a standardazied way. On top of all that,you always need to cope with net drive assignments, virtual net drivesthat will provide access to the underlying OS from inside your virtualXP and that behave a lot different in XP than Windows 7, as well assome restrictions regarding the virtual desktop or virtual windows theVirtualPC software provides. For example, you won't be able to move theemulated REST 2009 windows to a second monitor.
Afterall this complaining about how bad this free functionality in Windows 7is, I want to present my workaround that has since then worked verywell for me.
Downloadsneeded
- You will need the following.
- REST 2009installer
REST 2009 needs to be installed OUTSIDE of the virtual machine, i.e. ina regular manner
alternativeDownload link - CorFlags.exe
which may be obtained by installing the following.NETSDK 3.5. subcomponent(youneed to install the .NET Development tools compnent only)
PatchingREST 2009
Theproblem with REST 2009, which was most probably written in .NET is,that the author missed to flag the code as 32bit code. What happens,when executing undefined code under a 64bit system is, that the code isexecuted as 64bit and thus fails. Under a 32bit system, the 32bit codeworks fine.
UsingCorFlags.exe, it ispossible to subsequently edit those header flags, making a 64bitWindows able to correctly identify and interpret the code.
Afterthe above mentioned software components are installed,CorFlags.exeshould reside in
whereasRest.exeis located at
%PROGRAMFILES%Microsoft SDKsWindowsv6.1bin
whereasRest.exeis located at
%PROGRAMFILES(X86)%REST 2009
Usingan elevated command prompt (hit the WINDOWS key, search for cmd.exe,right-click cmd.exe and select 'Run as administrator...'), simply enterthe following command (or paste the code using the right mouse button):
If there is no error message appearing in your command prompt,everything worked as expected and you might try running theRest.exedirectly under your 64bitWindows installation.
cmd.exe /c '%PROGRAMFILES%MicrosoftSDKsWindowsv6.1binCorFlags.exe' '%PROGRAMFILES(X86)%REST2009Rest.exe' '/32bit+' & pause
If there is no error message appearing in your command prompt,everything worked as expected and you might try running theRest.exedirectly under your 64bitWindows installation.
![Rotor-gene Q Software Rotor-gene Q Software](/uploads/1/2/5/8/125877523/925825232.jpg)
Thisinstruction is copyright by Christian Wolf (2015) and may not bereproduced without written permission, instead you may freely link tothis page to help others coping with the same problem.Last updated: 27.05.2015